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. 2015 Aug 12;35(32):11266–11280. doi: 10.1523/JNEUROSCI.0779-15.2015

Figure 7.

Figure 7.

Zic2 thalamus–prethalamus INs are born between E11.5 and E13.5. A, Double staining for BrdU and Zic2 in coronal sections from E14.5 embryos with mothers that were injected with BrdU at E11.5 or E12.5. B, Quantification of the number of double-labeled Zic2/BrdU cells in E14.5 embryos from mothers injected with BrdU at E10.5, E11.5, E12.5, or E13.5. The majority of the Zic2 cells in the thalamic prethalamic boundary (TpTB) area were born between E11.5 and E12.5. Quantifications were performed in at least four sections per embryo. Numbers in the columns represent number of embryos analyzed. C, C′, Time-lapse sequence in the MS of E13.5 Tg(Zic2eGFP) embryos. At the left, color arrowheads point out the starting (top) and the ending (bottom) points of the video. At the right, the three examples of migrating eGFP cells (turquoise, violet, and green arrowheads) over time demonstrate that these cells move from lateral to medial locations. D, Dorsal; V, ventral; M, medial; L, lateral. Scale bars: A, C–C′, 100 μm; small panels, 20 μm.