Figure 6.
Pharmacological stimulation of α1 subunit-containing GABAA receptors in adult ME mice partially reduces the adult-specific extensive reactivation and decreases somatosensory cortex activation. A, Design of the pharmacological experiment in adult (P120) mice in relation to the weeks after ME. B, Circadian locomotion activity of sham (black) and indiplon-injected (gray) mice is depicted for day 1. C, Comparison of the total counts of beam crossings (mean activity) at day 1 up to the end of the period (day 20) revealed hypoactivity at the locomotion level in the indiplon group (gray), confirming its effectiveness in vivo. Cortical activity patterns as measured by means of ISH for the activity reporter zif268 (D–K) unraveled a significant decrease in visual cortex reactivation across the infragranular layers of the medial (J) and lateral monocular (H) cortices, as well as a significant drop in activity across both the upper (G) and lower (K) layers of somatosensory area S1.