Table 10.

List of primers used for cDNA amplification of rat and mouse Npsr1 cDNA sequences with additional FLAG tag

Species, gene, designation	Orientation	Primer sequence (5′ → 3′)
Rat, Npsr1, cDNA	(+)	CATGCTCGAGGCCACCATGCCGGCCAACCTCACAGAGGGCA
Rat, Npsr1, cDNA with FLAG	(−)	CATGGGATCCTTACTTGTCGTCATCGTCTTTGTAGTCGATGAATTCAGGCTTGGAGA
Mouse, Npsr1, cDNA	(+)	CATGCTCGAGGCCACCATGCCAGCCAACCTCACAGAGGGCA
Mouse, Npsr1, cDNA with FLAG	(−)	CATGGGATCCTTACTTGTCGTCATCGTCTTTGTAGTC GATGAATTCCGGCTTGGAGA
–, –, T7	(+)	TAATACGACTCACTATAGGG
–, –, Bgh	(−)	TAGAAGGCACAGTCGAGG