Table 3.
List of primers used for sequencing of the rat Npsr1 gene with location and exons/promoter/downstream enhancing region the primers hybridized to
| Sequential order | Location | Orientation | Primer sequence (5′ → 3′) |
|---|---|---|---|
| 1 | Intron 1 | (+) | GGTGAGCAATAGCCAGAAGC |
| 1 | Intron 2 | (−) | CAGAATTTAAAGCCAGGGCA |
| 2 | Intron 2 | (+) | GCGCAAGTGACTGTGTCATC |
| 2 | Intron 3 | (−) | CTTCTCTCCCGCTGGTACTG |
| 3 | Intron 3 | (+) | TCCATGCCTCACTTTTCCTC |
| 3 | Intron 4 | (−) | AGCTAGGGAGAAAGGCGTGT |
| 4 | Intron 4 | (+) | AGCCCAGATCTGCTTCCAGT |
| 4 | Intron 5 | (−) | ATGGCGTGAGGATCAGGTAG |
| 5 | Intron 5 | (+) | GTCCTAGTGACTCCCAGCCA |
| 5 | Intron 6 | (+) | GTTCCCACAAGGAGTTTGGA |
| 6 | Intron 6 | (−) | TGGCACCTTCAGTATGAGCA |
| 6 | Intron 7 | (+) | CCAGATACCCCTATTTCCAGC |
| 7 | Intron 7 | (−) | AGCCGCCACTAATCCATCTT |
| 7 | Intron 8 | (+) | ACACTCCTTCCCTGCATGTC |
| 8 | Intron 8 | (+) | AAGAGGGATGCTTCTGGTGA |
| 8 | Intron 9 | (−) | GAGCATTGGGAGCACAACTT |
| 9 | Intron 9 | (+) | TGGAGGAAGAGGTCCAGTTG |
| 9 | Exon 10 | (−) | ATGGTGAAGGTCTGGGTGAG |
| 10 | Exon 10 | (+) | CTCTCCAAGCCTGAATTCATC |
| 10 | Exon 10 | (−) | CTAACATCTTCTCCTCCACATG |
| 11 | Exon 10 | (+) | GGAGGACAACAAAGGTTAGAC |
| 11 | Exon 10 | (−) | ATAAGACCAGCACTTCCTTG |
| 12 | Exon 10 | (+) | AAATAGTGATAGACCCTGGC |
| 12 | Exon 10 | (−) | ACATGTTAACGACTGAACGA |
| 13 | Exon 10 | (+) | CCCACAGCCCTATGACGCACG |
| 13 | Exon 10 | (−) | TGCTAGCTAGGACACCCGCCA |
| 14 | Exon 10 | (+) | GCTGACGGCTCGTTCAGTCG |
| 14 | Exon 10 | (−) | AGGGGATGGTGTCGGCATGTG |
| 15 | Exon 10 | (+) | ACAGGACTGGTGCTGAAATCGC |
| 15 | Exon 10 | (−) | ACTTCAACATCCTCTGCTACACTGC |
| 16 | Exon 10 | (+) | GTCCTATGATGCTGGATGAATCATGC |
| 16 | Exon 10 | (−) | CCTGAAAGGAGAGGATCTTTCGCCA |
| 17 | Exon 10 | (+) | GGTGCCCACCTTCCACACCAAGATG |
| 17 | DER | (−) | GGCCATCAGACGTGTGGCTTCC |
| 18 | DER | (+) | CCAGCTTCATAGAGACAGCTCTGC |
| 18 | DER | (−) | ACCCCCATTCTCCCACCCCAC |
| 19 | DER | (+) | TCATTATCCACAACAGGGCTGGACC |
| 19 | DER | (−) | ATGGCCTGCAAGGCTAAGGCG |
| 20 | DER | (+) | CAGCACTTGGGAGACAGAGA |
| 20 | DER | (−) | TGCTGAGCTAAATGTCAAAG |
| −1 | Promoter | (+) | AGACAAACACAGACCCCTGC |
| −1 | Intron 1 | (−) | GAGTTCAGTTAGCCAGGGCA |
| −2 | Promoter | (+) | TGTCATGTCGAAACCCTTCA |
| −2 | Promoter | (−) | CAGCTGAGATCGCTTTTGTCT |
| −3 | Promoter | (+) | GTCAGCAGCTTCTGTGCATC |
| −3 | Promoter | (−) | AAGGGGTATGTCCCAGGAAG |
| −4 | Promoter | (+) | TGCACCCATTTTTAGTTCCC |
| −4 | Promoter | (−) | AGGGGTATGTCCCAGGAAGT |
| −5 | Promoter | (+) | GATCCTACTTTGGGCCTGTCG |
| −5 | Promoter | (−) | GAAGATGCTCAACCACATTATTAGC |
| −6 | Promoter | (+) | GTCCACCCCTGAGAGTTCCAG |
| −6 | Promoter | (−) | CAGGGCATCAAGTGAGGGCATC |
| −7 | Promoter | (+) | GGTGTGGATTTGTGAGGGAGGT |
| −7 | Promoter | (−) | GTTCAGTCAGGGAAGATGCATC |
DER, Downstream enhancing region.