Table 5.
List of primers used for sequencing of the rat Nps gene with location and exons/introns/promoter/downstream enhancing region the primers hybridized to
| Sequential order | Location | Orientation | Primer sequence (5′ → 3′) |
|---|---|---|---|
| 1 | Promoter | (+) | CCCCTGGCCACCCATGTCAC |
| 1 | Exon2, Intron 2 | (−) | AGCCGTGAAGCCCTTACCTTGGA |
| 2 | Promoter | (+) | GCAGGCTCAGACAGCGAGCG |
| 2 | Intron 2 | (−) | GAAACAGCCATTTCCATGTGCAGG |
| 3 | Intron 2 | (+) | TCAGGATGGTGGAGTGCCCAA |
| 3 | Intron 2 | (−) | GCTCATGGCATAGGAGCAAGGACA |
| 4 | Intron 2 | (+) | AAATGATTGCCTTTCTTCGGGGGT |
| 4 | Intron 2 | (−) | ACACCACCTTGTGGCCCAGGA |
| 5 | Intron 2 | (+) | TCCAAGTGGCAACTCCTGCAAGC |
| 5 | Intron 2 | (−) | AGGCAGCACCATCGCTCACC |
| 6 | Intron 2 | (+) | TGTCCCTAAAGGTTTGCTCACCGC |
| 6 | Intron 2 | (−) | ACTGCCCATTTTAAGTCTTGAGCCACC |
| 7 | Intron 2 | (+) | AGTGGCCTCTGGGAAGAGTGG |
| 7 | Intron 2 | (−) | GCCCTGGCTGAGTGAATGACTGG |
| 8 | Intron 2 | (+) | TGCACATCTTCTTTCCTCCAGAGCCA |
| 8 | Intron 2 | (−) | GCCTCCGATGGGAGCTGCTG |
| 9 | Intron 2 | (+) | TCCCAACCCCCAAACAGAGCG |
| 9 | Intron 2 | (−) | ACCGGGCCAAAGGAACCTGC |
| 10 | Intron 2 | (+) | TGGCTCTGGCGCTTGGCTTC |
| 10 | Intron 2 | (−) | AGCCCTAGGTTTAGCCCCCAGC |
| 11 | Intron 2 | (+) | CGGCCTGCCCATGCACACTTA |
| 11 | Exon 3 | (−) | GCCTGGCTGGGCAGGTACTC |
| 12 | Intron 2 | (+) | GCTGTGTTTCAGTGATGTTTCTCCCCA |
| 12 | DER | (−) | GGCGGAAGTTTGAGACAGGTTTGC |
| 13 | Exon 3 | (+) | ACGGAGTCGGCTCAGGGGTG |
| 13 | DER | (−) | CGCTGGCGATCCCTTGCTGC |
| 14 | DER | (+) | ACGACGCGTGGGCGTTTCTAC |
| 14 | DER | (−) | TGACCTGGCAGGGACAGCGA |
| 15 | DER | (+) | CCTGGGTCTGTTTCTCCCCCTC |
| 15 | DER | (−) | CTGGAAGCTGGTGCCAAGGATAC |
| −6 | Promoter | (+) | GGAGCTGCAGGCAAAGCCTCA |
| −6 | Promoter | (−) | ACCCAAACCAAGGTTCCTCACCA |
| −5 | Promoter | (+) | GATACAGCAAACAGGAGGGA |
| −5 | Promoter | (−) | TCTCCAAAGAACAGAGCTCC |
| −4 | Promoter | (+) | CAAGAAGAAGGGAAGTGATGTGGCA |
| −4 | Promoter | (−) | AGGACAAGGAGGTGACCCAGCT |
| −3 | Promoter | (+) | CCCAGGCTTCCAGCTTGGCA |
| −3 | Promoter | (−) | CGGCAGAGGAAAACGTCAGAGGG |
| −2 | Promoter | (+) | CGGATCCTTGTGCTTGCATGGC |
| −2 | Promoter | (−) | GGCCAGGGGCCTCCAAAGGA |
| −1 | Promoter | (+) | CAGCCCTGTCAGCCTGCATCA |
| −1 | Intron 1 | (−) | AGGACCTTGGGTGGGATCTCACAC |
DER, Downstream enhancing region.