Figure 6.

Galanin and NPY receptor binding and functional binding in the human epileptic hippocampus and amygdala. A, Galanin and (B) NPY receptor binding in the human epileptic hippocampus. C, D, Nonspecific binding corresponding to A and B, respectively. E, Hematoxylin staining of an adjacent section showing the gross morphology of the layers analyzed. F, [¹²⁵I]-galanin binding (top left), corresponding nonspecific binding (bottom left), [¹²⁵I]-PYY binding (top right), and corresponding nonspecific binding (bottom right) in sections from the human amygdala. G, Galanin and NPY(H) receptor functional binding. I, Basal and nonspecific (J) binding corresponding to G and H, respectively. K, Hematoxylin staining of an adjacent section showing the gross morphology of the layers analyzed. L, Galanin functional binding (top left), NPY functional binding (top right), and corresponding basal binding (bottom left) in sections from the human amygdala. M, Quantification of specific [¹²⁵I]-galanin and [¹²⁵I]-PYY receptor binding measured in hippocampal regions (n = 5) and amygdala (n = 2). N, Quantification of galanin and NPY receptor functional binding (i.e., peptide-stimulated binding minus basal binding) measured in hippocampal regions (n = 5) and amygdala (n = 2). Note almost complete absence of galanin receptor functional binding signal (N), despite specific [¹²⁵I]-galanin binding found in M. Mol, stratum moleculare; rad, stratum radiatum; lac-mol, stratum lacunosum moleculare. Scale bars: A–E, G–K, 3 mm; F, L, 4 mm.