Figure 2.

The excitatory effect of AVP is unchanged around the expected time of the LH surge. A, B, Timing of experiments in proestrus and diestrus. Mice were killed at the end of the light cycle (ZT10, arrow). Recordings were undertaken between ZT11 and ZT14. The expected time of the preovulatory LH surge occurring in proestrous mice is indicated in A. C, D, Example traces and corresponding rate meters (20 s bins) illustrating the effect of AVP on RP3V kisspeptin neuron spontaneous firing around the expected time of the surge in proestrus and in a time-of-day control diestrous mice. E, Summary bar graph of the net effect of 1 μm AVP on RP3V kisspeptin neuron firing in diestrous and proestrous females killed hours before (ZT4–ZT6) or just before (ZT10) the end of the light cycle. Bars for ZT4–ZT6 are from Figure 1F.