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. 2015 Aug 19;35(33):11500–11513. doi: 10.1523/JNEUROSCI.0343-15.2015

Figure 3.

Figure 3.

Levels of Aβ peptides are reduced in ADINKO cortex. A–D, Aβ40 and Aβ42 in cortical homogenates of 16-month-old (A, B) and 23-month-old (C, D) ADINKO mice and controls (n = 6–12). FA-treated insoluble and DEA-soluble fractions measured by human-specific ELISA. E, Western immunoblot (WIB) analysis of human APP (hAPP), CTF-α/β, human Aβ (hAβ) from cortical homogenates revealed diminished Aβ monomer levels with no change in APP processing. F, SDS-stable Aβ oligomers were extracted from cortex as detergent-soluble (left blot) and extracellular-enriched (right) fractions, and submitted to WIB using anti-Aβ antibody 6E10 and Odyssey detection (LI-COR). Specific bands migrated at 8 kDa (D, dimers), 12 kDa (T, trimers) and 18 kDa (4-mers or 5-mers; HM, high-molecular-weight oligomers; Mc Donald et al., 2010) and were diminished in ADINKO mice. M, Monomers. *p < 0.05, **p < 0.01, ***p < 0.001. Mean ± SEM, Mann–Whitney U test, n = 5 mice.