Table 5.
A summary of targets of Sox9a and Atoh1a
| Sox9a targets (gene/location) | Atoh1a targets (gene/location) | |
|---|---|---|
| Verified, targets of both | bnc1/-8217bp | bnc1/-1331bp |
| eya2/ 1st intron | eya2/-39376bp | |
| fn1/-14566bp | fn1/-26089bp | |
| neo1/-1010bp | neo1/1st intron | |
| spry1/-3886bp | spry1/ 5′UTR | |
| tlx2/1st intron | tlx2/-4239bp | |
| zgc77614/-4658bp | zgc77614/-187bp5 | |
| Verified, targets unique to one | dacha/ 2nd intron | dlc/-2774bp |
| cx43/-16069bp | irx2a/-8618bp | |
| tbx1/1st intron | jag1b/1st intron | |
| unc5b/1st intron | notch1b/-7642bp | |
| Not verified | cdh2/-4914bp | cdh2/-32746bp |
| irx1a/-29014bp | dacha/1st intron | |
| notch1b/2nd intron | irx1a/-36793bp | |
| prdm1a/-1668bp | irx5a/1st intron | |
| robo1/1st intron | pax2b/-18427bp | |
| spry2/-31106bp | pou3f3b/-27665bp | |
| spry4/4th intron | prdm1a/4th intron | |
| spry2/-16082bp | ||
| spry4/-27068bp | ||
| unc5b/1st intron |
All of these genes are relevant to the otic development. By performing ChIP-PCR assay, we verified 11 of 21 genes for Atoh1a and 11 of 18 genes for Sox9a. Among verified genes, seven are common to both sets.