A, Photomicrographs showing representative c-Fos-IR for a vehicle-injected animal. B, Photomicrographs showing representative c-Fos-IR for an animal injected with CNO (3 mg/kg, i.p.). C, Example dual-labeled c-Fos-IR/GFP-IR neurons in dmPFC, D, Counts of total c-Fos-IR and total c-Fos-IR/GFP-IR dual-labeled neurons. White arrowheads show single-labeled c-Fos-IR, and yellow arrows show dual-labeled c-Fos-IR/GFP-IR neurons. Scale bar, 25 μm. *p < 0.05 versus vehicle group. Group sizes: hSyn-hM3Dq-CNO, n = 4; hSyn-hM3Dq-vehicle, n = 5.