Figure 5.

BBB permeability is not involved in pain modulation by Cav-1 in ACC. A, The increased expression of t-Cav-1 in contralateral ACC by CCI and ipsilateral ACC by Lenti-Cav-1 was not colocalized with CD34 as markers of endothelial cells (n = 5). Scale bar, 50 μm. B, Quantification of EB leakage at day 7 after CCI showing no increased extrinsic EB content in contralateral ACC to the injured side (n = 5). C, Quantification of EB leakage at day 7 after CCI showed that overexpression of Cav-1 in ipsilateral ACC to the injured side also could not increase extrinsic EB content (n = 5). Data are shown as means ± SEM. EB, Evan's blue.