Figure 7.

Bex3 increases the mouse trkA gene promoter activity and binds close to the transcriptional start site. A, Mapping the region of the mouse trkA promoter modulated by GFP-Bex3. The transcriptional activities of different reporter plasmids containing different sequences from the 5′ region of the mouse trkA promoter (top) are shown. Data are presented as the mean ± SEM. Data are reported as GFP versus GFP-Bex3, respectively (100% vs 188.7 ± 16.9%, for pMS8; 99.95 ± 28.75% vs 214.8 ± 19.01%, for pMS10.3; 113.7 ± 28.75% vs 196.2 ± 20.49%, for pMS16; 14.05 ± 2.2% vs 16.54 ± 3.17%, for pMS22; n = 4–9 using duplicates). The p values were calculated using a paired two-tailed Student's t test. B, Endogenous Bex3 protein binds to the mouse trkA promoter in N2a cells. ChIP analysis of N2a cell lysates using anti-Bex3-ProtG-agarose. Goat IgG-ProtG-agarose was used as a control. A representative experiment is shown (n = 5). C, Quantitative ChIP assays in PC12 cells. Cell lysates were immunoprecipitated using the anti-Bex3-ProtG-agarose or goat IgG-ProtG-agarose as a control. qPCRs were performed using the oligonucleotides described in Materials and Methods. The p value was calculated using a two-tailed Student's t test (n = 3 by triplicate).