Figure 6.

Distribution and function of NGL-2▵PB in hippocampal neurons. A, Hippocampal neurons were transfected with pEGFP-NGL-2▵PB. After 24 h (stage 2) or 48 h (stage 3), cells were fixed and stained with neuron marker Tuj1. Scale bar, 25 μm. B, Profiles of NGL-2▵PB immunofluorescence intensity in the processes of stage 2 and stage 3 neurons. Fluorescence intensities were plotted against the distance from the soma, as shown in A. C, The effect of NGL-2▵PB overexpression on axon differentiation. Hippocampal neurons were transfected with pEGFP-NGL-2▵PB or pEGFP-empty vector. Transfected neurons were identified by EGFP and stained with axonal marker Tau1. Scale bar, 25 μm. D, Quantitative analysis of axon differentiation transfected by NGL-2▵PB. N.S., Not significant (Student's t test).