Figure 2.
BMM differentiation in the presence of POSs. A–C, Quantitative RT-PCR of Cd206 (A), Il-1Ra (B), and P2rx7 (C) mRNA normalized with Rps26 mRNA of C57BL/6J- and Cx3cr1GFP/GFP-BMMs cultured for 18 h with or without POSs (n = 5 per group, *p < 0.01; one-way ANOVA followed by Bonferroni's post-test, representative of 3 independent experiments). D, Quantification of ATP release from C57BL/6J- and Cx3cr1GFP/GFP-BMMs cultivated for 18 h with or without POSs (n = 6 per group, *p < 0.01; one-way ANOVA followed by Bonferroni's post-test, representative of 3 independent experiments). E, Quantitative RT-PCR of Il-1β mRNA normalized with Rps26 mRNA of C57BL/6J- and Cx3cr1GFP/GFP-BMMs cultured for 18 h with or without POSs (n = 4 per group, *p < 0.01; one-way ANOVA followed by Bonferroni's post-test). F, Quantitative RT-PCR of Il-1β mRNA normalized with Rps26 mRNA of C57BL/6J- and Cx3cr1GFP/GFP-BMMs cultured for 18 h with or without POSs and LPS (60 ng/ml; n = 4 per group). G, Quantification by ELISA of IL-1β in the supernatants of C57BL/6J- and Cx3cr1GFP/GFP-BMMs cultured for 18 h with or without POSs and stimulated or not with LPS (60 ng/ml) for 2 h (n = 4 per group, *p < 0.01; one-way ANOVA followed by Bonferroni's post-test).