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Representative image of double-labeled immunohistochemistry detecting Fos and CaMKII (A), Fos and TH (B), and Fos and GAD67 (C) associated with nicotine-induced acceleration of the escalation of alcohol self-administration. Green-labeled nuclei indicate Fos expression, while red-labeled cells indicate CaMKII, TH, and GAD67 labeling in panels A, B, and C, respectively. Scale bar, 100 μm. n = 4 per group.
