Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 12;44(2):630–642. doi: 10.3892/ijmm.2019.4240

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Downregulation of XIST decreases extracellular matrix degradation in AC/OA and AC/IL-1β-5.0 cells. (A) Downregulation of XIST by an RNA interference assay was confirmed by RT-qPCR. MMP-13 expression decreased following knockdown of XIST, as determined by (B) RT-qPCR, (C) western blot analysis (lysates were analyzed by immunoblotting with MMP-13 and GAPDH antibodies; the left panel presents data from 3 independent experiments) and (D) immunofluorescence analysis. The knockdown of XIST also downregulated the expression of ADAMTS5 as determined by (E) RT-qPCR, (F) western blot analysis (lysates were analyzed by immunoblotting with ADAMTS5 and GAPDH antibodies; the left panel presents data from 3 independent experiments) and (G) immunofluorescence analysis. All data were normalized to the negative control shRNA group. Magnification, ×100; scale bar, 100 µm. The error bars represent standard deviation (n=3). ^**P<0.01. XIST, X-inactive-specific transcript; AC, articular chondrocyte; OA, osteoarthritis; IL-1β, interleukin-1β; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; MMP-13, matrix metalloproteinase 13; ADAMTS5, ADAM metallopeptidase with thrombospondin type 1 motif 5; NC, negative control; sh, short hairpin.

Downregulation of XIST decreases extracellular matrix degradation in AC/OA and AC/IL-1β-5.0 cells. (A) Downregulation of XIST by an RNA interference assay was confirmed by RT-qPCR. MMP-13 expression decreased following knockdown of XIST, as determined by (B) RT-qPCR, (C) western blot analysis (lysates were analyzed by immunoblotting with MMP-13 and GAPDH antibodies; the left panel presents data from 3 independent experiments) and (D) immunofluorescence analysis. The knockdown of XIST also downregulated the expression of ADAMTS5 as determined by (E) RT-qPCR, (F) western blot analysis (lysates were analyzed by immunoblotting with ADAMTS5 and GAPDH antibodies; the left panel presents data from 3 independent experiments) and (G) immunofluorescence analysis. All data were normalized to the negative control shRNA group. Magnification, ×100; scale bar, 100 µm. The error bars represent standard deviation (n=3). ^**P<0.01. XIST, X-inactive-specific transcript; AC, articular chondrocyte; OA, osteoarthritis; IL-1β, interleukin-1β; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; MMP-13, matrix metalloproteinase 13; ADAMTS5, ADAM metallopeptidase with thrombospondin type 1 motif 5; NC, negative control; sh, short hairpin.