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. 2019 Jun 6;44(2):535–548. doi: 10.3892/ijmm.2019.4233

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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ORMDL3 promotes SPHK1 activation in 16HBE cells. SPHK1 protein expression levels in (A) ORMDL3/Vector and (B) si-ORMDL3/si-control 16HBE cells, detected by western blotting. SPHK1 mRNA expression levels in (C) ORMDL3/Vector and (D) si-ORMDL3/si-control 16HBE cells, detected by quantitative PCR. Enzyme activities of SPHK1in (E) ORMDL3/Vector and (F) si-ORMDL3/si-control 16HBE cells, detected with SPHK1 Activity Assay kit I. Immunofluorescence staining was used to assess SPHK1 localization in (G) ORMDL3/Vector and (H) si-ORMDL3/si-control 16HBE cells. Values are the mean ± standard deviation (n=6 per group in duplicate). ^*P<0.05 and ^**P<0.01 vs. the corresponding control group. SPHK1, sphingosine kinase 1; ORMDL3, orosomucoid-like protein isoform 3; si, small interfering; Ctrl, control.