Table II.

Primers used in quantitative PCR for mouse lung assessment.

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)
IL-4	TCTCGAATGTACCAGGAGCCATAT	AAGCACCTTGGAAGCCCTACAGA
IL-13	GCAGCATGGTATGGAGTGTG	CCTCTGGGTCCTGTAGATGG
TNF-α	CTGGATGTCAATCAACAATGGGA	ACTAGGGTGTGAGTGTTTTCTGT
Claudin-18	GACCGTTCAGACCAGGTACA	GCGATGCACATCATCACTC
E-cadherin	CAGGCTGGCTGAAAGTGACA	ACGGATCCCTCAAACACCTC
SPHK1	CATCACGGCCTGTAAAAAGGT	ATCTTCCACAAACCCAATCTGG
GAPDH	AATGGATTTGGACGCATTGGT	TTTGCACTGGTACGTGTTGAT

E, epithelial; SPHK1, sphingosine kinase 1; IL, interleukin; TNF, tumor necrosis factor.