Table 1.
Antibody list
| Antigen | Reference | Dilution | Protocol |
|---|---|---|---|
| B220 | Rat anti-mouse CD45R RM2600 (Life Technologies) | 1:50 | Paraffin section |
| CD3 | Rabbit anti-human A0452 (Dako) | 1:50 | Paraffin section |
| Gr1 (LY6C/G) | Rat anti-mouse mAb (clone RB6-8C5) RM3030 (Life Technologies) | 1:100 | Paraffin section |
| Iba1 | Rabbit polyclonal 019-19741 (Wako) | 1:400 | Paraffin section |
| 1:400 | Fixed section | ||
| Gfap | Mouse anti-mouse (G3893) or rabbit anti-human (G9269) (Sigma) | 1:500 | Fixed or frozen section |
| MHC II | Anti-mouse MHC II (I-A/I-E) APC, clone MS 114.15.2 (Affymetrix eBioscience) | 1:1000 | Fixed section |
| IgG | Goat anti-mouse Alexa Fluor-conjugated A11029 or A21424 (Life Technologies) | 1:2000 | Frozen section |
| S100 | Rabbit S2644 (Sigma) | 1:1000 | Fixed section |
| Occludin | Rabbit anti-human 71-1500 (Life Technologies) | 1:500 | Western blot |
| ZO-1 | Mouse anti-human 339100 (Life Technologies) | 1:500 | Western blot |
| Claudin-5 | Rabbit anti-mouse 34-1600 (Invitrogen) | 1:100 | Western blot |
| GAPDH | Mouse anti-rabbit G9295 (Sigma) | 1:5000 | Western blot |
| CD3 | Armenian hamster anti-mouse PE-coupled (clone 1452c11) 100308 (Biolegend) | 1:200 | FACS |
| CD4 | Rat anti-mouse adenomatous polyposis coli–Cy7-coupled (clone GK1.5) 100404 (Biolegend) | 1:800 | FACS |
| CD11c | Rat anti-mouse adenomatous polyposis coli-coupled (clone H65) 561115 (BD Biosciences) | 1:200 | FACS |
| CD8 | FITC rat anti-mouse CD8a (clone 53-6.7) (BD Biosciences) | 1:200 | FACS |
| CD11b | Anti-mouse/human PE-Cy7-coupled (clone M1/70) 101216 (Biolegend) | 1:800 | FACS |
| C1q | Rabbit anti-C1qA (1160-1-AP) (Proteintech) | 1:500 | Western blot |
| C9Neo | Rabbit anti-rat C9 (provided by Prof. B. Paul Morgan, Institute for Infection and Immunity, University of Cardiff, UK) | 1:500 | Western blot |
| Vwa5a | Rabbit anti-human, mouse Vwa5a C-terminal region (Clinisicences) | 1:50 | Fixed cells |