Figure 6.
Further characterization of the lactate-permeable channel. A, Traces obtained with a lactate-rich (145 mm), chloride-low (10 mm) medium, with pipette in the cell-attached mode at 120, 100, 60, and 0 applied potential. B, Single-channel current obtained at increasing nominal patch potentials (calculated assuming an astrocytic membrane potential of −80 mV). The equilibrium potentials estimated for lactate and chloride are indicated. C, A cell was sequentially bathed with 10 mm lactate and 3 mm Ba2+ for periods of 5 min as shown.