Figure 1.

Micro-CT of cuprizone-treated mouse brains. A, B, Coronal plane reconstruction of micro-CT scans of Histodenz-immersed brains derived from wild-type (+/+) and Ptprz-deficient (−/−) mice. Images show the whole region (A) and dorsal corpus callosum corresponding to the square region in A (B). The CT number increases from black to white. Mice were fed a cuprizone-containing diet for 6 weeks (0) to induce demyelination, followed by spontaneous remyelination with the removal of cuprizone (1, 3, and 5 weeks). Normal mice were maintained on the regular diet. Scale bars, 1 mm. C, Scatterplot showing differences between the CT number of the dorsal corpus callosum and that of the cerebral cortex (each circle corresponds to an individual mouse). Open circles, Wild-type mouse; closed circles, Ptprz-deficient mouse. n = 10 for each group. *p < 0.05, **p < 0.01, two-way ANOVA with Bonferroni post hoc test.