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. 2015 Apr 8;35(14):5707–5723. doi: 10.1523/JNEUROSCI.4115-14.2015

Figure 3.

Figure 3.

Depletion of dendritic mitochondria inhibits dendritic outgrowth in vivo Purkinje cells and in vitro hippocampal neurons. A, Dendritic morphology of Purkinje cells. Cells were transfected with tdTomato (green) with or without T20-GFP-GM130Cterm at E12.5 and fixed at P14 for Calbindin immunostaining (magenta). Scale bars: 10 μm. B, Quantification of the total sagittal-sectional area of the molecular layer covered by the dendrites. N ≥ 20 for each data point: ***p < 0.001, Student's t test. Data represent means ± SEM. C, D, Cultured hippocampal neurons were transfected with tdTomato (magenta) and Mito-EGFP (green) together with T20-myc-GM130Cterm at 3 DIV, and stained with anti-myc antibody at 7 DIV. Each part shows the representative morphology of neurons (C) and distribution of mitochondria in dendrites (D). Scale bars: 10 μm. E, F, Quantification of total dendrite length (E) and dendritic branch distribution (F) in 7 DIV hippocampal neurons. N = 10 for each data point: **p < 0.01, Student's t test. Data represent means ± SEM.