Figure 1.

Distinct intrinsic networks measured with O₂ amperometry at rest. a, O₂ probes targeted two regions of the DMN (PRL and RSC) and two regions of the LCN (M2 and S1J). b, Final placements of O₂ probes based on histological analysis. c, The network connectivity pairs obtained. d, Raw regional O₂ signals filtered between 0.01 and 0.1 Hz from one representative animal over 5 min at rest. Left panel shows within-network regions of the DMN (PRL and RSC) with highly synchronous fluctuations. Right panel shows between-network regions (RSC and M2) with uncorrelated low-frequency fluctuations. e, Correlation matrix of functional connectivity (r) during unscheduled spontaneous behavior. f, Bar chart of connectivity of each node pair, mean r ± SEM, n = 19. Significant between-network versus within-network pair difference (***p < 0.0001).