Figure 2.

Morphology and intrinsic properties of layer 3 principal neurons in the MEC. A, Maximal intensity projection of a confocal image stack of an exemplary layer 3 pyramidal cell in the MEC. The shown cell was filled with biocytin during electrophysiological recording and counterstained with streptavidin A647 after fixation. Scale bar, 100 μm. B, Representative electrophysiological characterization of a layer 3 pyramidal cell. Hyperpolarizing and depolarizing current steps were applied to determine passive properties and action potential pattern. C, Summary table of intrinsic membrane properties of layer 3 pyramidal cells. D, Distribution of intrinsic parameters for all recorded cells. Gray circles represent mean values of individual neurons; black lines are mean values of all neurons (n = 23).