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. 2015 Sep 9;35(36):12346–12354. doi: 10.1523/JNEUROSCI.0696-15.2015

Figure 3.

Figure 3.

Layer 3 dendrites avoid calbindin-positive pyramidal cell patches in layer 2. A, Sagittal section through the MEC stained for calbindin (green, left) and PCP4 (red, middle). Right, Overlay. Note the clustering of apical layer 3 dendrites around the calbindin patches in layer 2. Scale bar, 500 μm. B, Close-up views from a tangential section through superficial MEC layer 2, showing neuropil signal of retrogradely labeled layer 3 neurons, following injections of CTB-Alexa 488 (green) and CTB-Alexa 546 (red) as in Figure 1C, stained for calbindin (gray). Arrowheads indicate calbindin patches. C, Reconstructions of the apical dendritic morphologies of three representative layer 3 pyramidal neurons, superimposed on the calbindin staining in MEC layer 2 (tangential view). Note that the dendrites largely avoid the calbindin territories. Scale bar, 200 μm. D, Left, Tangential section through layer 3 of the MEC stained for neurobiotin (red) and calbindin (green) showing the site of neurobiotin “spillover” in MEC layer 3, where a cluster of layer 3 neurons is labeled (arrow). Right, A close-up magnification on the apical dendrites: calbindin patches (green, top), neurobiotin-labeled (Nb) dendrites (middle, red), and overlay (bottom). Dotted lines represent patch outlines. Note that the labeled dendrites (red) largely avoid the patch territories (green). Scale bars: left, 200 μm; right, 100 μm. D, Dorsal; L, lateral; M, medial; V, ventral.