Table 1.
PCR primers
| GenBank accession no. | First PCR primers | Size (bp) | Second PCR nested primers | Size (bp) |
|---|---|---|---|---|
| GAD65 (1) | PPM25176G-200 | 84 | PPM25176G-200 | 84 |
| NM 008078 | ||||
| GAD65 (2) | CCAAAAGTTCACGGGCGG, TCCTCCAGATTTTGCGGTTG | 375 | CACCTGCGACCAAAAACCCT, GATTTTGCGGTTGGTCTGCC | 248 |
| NM 008078 | ||||
| GAD67 (1) | PPM36500F-200 | 121 | PPM36500F-200 | 121 |
| NM 008077 | ||||
| GAD67 (2) | ATGATACTTGGTGTGGCGTAGC, GTTTGCTCCTCCCCGTTCTTAG | 253 | CAATAGCCTGGAAGAGAAGAGTCG, GTTTGCTCCTCCCCGTTCTTAG | 177 |
| NM 008077 | ||||
| SUR1 | CACCGCTGTTCCTGCTGTCT, CGCTCTGGGTTGCTTGTCCT | 523 | TCATCGGAGGCTTCTTCACC, TCTTCTCCCTCGCTGTCTGG | 208 |
| NM_011510.3 | ||||
| SUR2 | GCAGTCAAAGCCATCATCAGTGT, CAAGGATGGCAAGGAGAAGAG | 373 | CTTGAGCGATGAGATTGGTGAGG, AGATGATTTTCCACAACCCACTT | 310 |
| NM_001044720.1 | ||||
| Kir6.1 | GCTGGCTGCTCTTCGCTATC, CATACAGGGGGCTACGCTTG | 588 | TGGGTTTGGAGGGAGAATGA, TGGCGTCGTGGTTTTCTTGA | 286 |
| NM_008428.4 | ||||
| Kir6.2 | GGGTGGTAACGGCATCTTCC, ATGCTAAACTTGGGCTTGGC | 420 | CGCTCTACGACCTGGCTCCT, GGGTCAGGGCATCCAGCAG | 285 |
| NM_010602.2 | ||||
| GK (1) | GGCTGCAACGCCTGCTACAT, GCTCCTTGAAGCTCGGGTGC | 580 | GGGGCGCATGTGTGTCAACA, CACAATGTCGCAGTCGGCGA | 358 |
| NM_010292.4 | ||||
| GK (2) | PPM05095A-200 | 115 | PPM05095A-200 | 115 |
| NM 010292 | ||||
| GLUT3 | ATGGGGACAACGAAGGTGAC, GCCACAGAAAATGCCAATGA | 377 | CATCTCTGGTGTTCGCCGTG, CAGCATTTCAACAGACTCCGC | 338 |
| NM_011401.4 |