Figure 2.

Syt1-RQ mutant rescues docking in syt1 null cells. A, Ultrastructural images of different noninfected and infected conditions on the syt1 null cell background. Arrows mark docked vesicles. Scale bars: 200 nm. B, Normalized cumulative distribution of vesicles as a function of the distance from the plasma membrane. Inset, Distance in the range of 0–100 nm from membrane. C, D, Number of total (C) and docked (D) vesicles per section showing no difference in docking for Syt1-RQ mutant. ***p ≤ 0.001 by multilevel analysis (between 1–8 cells were collected from 5–8 embryos per condition; overall cell numbers and embryo numbers are annotated in C). Error bars indicate SEM.