Figure 8.

Syt1-RQ mutant overexpression in wild type chromaffin cells does not affect the IRP size. A, Mean membrane capacitance, Ca²⁺, and amperometry measurements of wild-type cells (blue), Syt1-wt overexpression (black), and Syt1-RQ mutant (red) overexpression in wild-type cells. B, There was no difference in IRP size (capacitance increase elicited by the first 6 brief 10 ms depolarizations) between conditions. C, D, RRP (capacitance increase elicited by the 6 10 ms depolarizations, and the 4 100 ms depolarizations) and total membrane capacitance after a Ca²⁺ uncaging flash were not significantly different in any condition. Error bars indicate SEM.