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. 2015 Oct 28;35(43):14501–14516. doi: 10.1523/JNEUROSCI.1056-15.2015

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Copyright © 2015 the authors 0270-6474/15/3514501-16$15.00/0

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Figure 6. — Luxol Fast Blue staining. A–H, Luxol Fast Blue staining of sagittal paraffin embedded brain sections of 60-d-old animals. Spongy degeneration and vacuoles formation go along with demyelination in Nat8L^+/+/Aspa^nur7/nur7 (B, F) mice. Demyelination is demonstrated by less intense Luxol Fast Blue staining (arrows in F and G) shown, for example, in the white matter of cerebellum (A–D) and corpus callosum (E–H) compared with control littermates (A, E). Nat8L^−/−/Aspa^nur7/nur7 (D, H) did not show any indication of myelin loss compared with controls. Scale bar, 200 μm.