Figure 5.

Knockdown of Luman expression decreases the injury-induced UPR. A, Volcano plot derived from qRT-PCR array data monitors UPR-associated transcripts with log2 fold change for injury-conditioned DRG neurons transfected with Luman-specific versus control-scrambled siRNA (x-axis) as a function of p value (y-axis) (horizontal dashed line, p = 0.05). Transcripts downregulated >2-fold are shown as solid spots with gene designations (p < 0.05). B, qRT-PCR array data were verified by qRT-PCR for mRNAs identified in A (N = 4 animals/condition as in A; N = 4 experimental replicates). C, qRT-PCR analysis of mRNA levels in 1 d injury-conditioned DRG neurons cultured 48 h in nontargeting control siRNA (Control) or Luman-specific siRNA (Luman Si) for markers assessed in Figure 1 as indicated (N = 3 experimental replicates). *p < 0.01.