Correct and incorrect MD cannula placement in the MnPO. Shown are Paxinos schemes (A, B) and Nissl staining (C, D) for determination of cannula placement. The sites where the infusion had an effect on Tb (•,○) and the areas where probe placements did not have any effect on temperature (x) are indicated in the schemes from Paxinos rat brain atlas. E, c-FOS-IR of a correct MD cannula placement (•,○) after V1Aant infusion. F, Time points when infusions were done; black bars indicate the dark phase and the white bar indicates the light phase; (1) indicates the day experimental setup and (2) the night experimental setup as described in the experimental procedures. AC, Anterior commissure; OC, optic chiasm.