Figure 7.

Cx43 hemichannel function increases extracellular ATP levels. A, Extracellular ATP levels measured by bioluminescence assays are shown for individual experiments (n = 4, 5, 5, and 5 in each condition, respectively). B, Group data and statistics. Blockade of Cx43 HCs with Gap26 in Cx30 KO (Ctrl) reduces ATP levels to a similar extent as Cx gene removal (dKO condition; p = 0.69, two-tailed Mann and Whitney U test; p < 0.001, one-way ANOVA followed by Newman–Keuls post hoc tests; *p < 0.05, **p < 0.01 compared to control without Gap26) but has no effect in dKO OB slices (^##p = 0.005, two-way ANOVA, interaction).