Fig. 3.

NOD2 and TLR-2 cross-regulated the expression of their respective adaptor molecules. (A) 29-kDa FN-f increased the protein levels of TLR-2 and NOD2 in a time-dependent manner. Chondrocytes were incubated with 29-kDa FN-f for 4, 8, 16, and 24 h. (B, C) NOD2 and TLR-2 receptors were involved in expression of the adaptor molecules of NOD2 and TLR-2. Chondrocytes were transfected with control siRNA and (B) si-NOD2 or (C) si-TLR-2. Forty-eight hours later, chondrocytes were stimulated with 29-kDa FN-f for 24 h. The protein levels were measured by immunoblot analyses. β-actin served as a loading control.