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. 2015 Jan 21;35(3):936–942. doi: 10.1523/JNEUROSCI.1998-14.2015

Figure 3.

Figure 3.

Vrk1 knockdown affects cell cycle in the developing mouse brain. A–D, Representative mouse brain slices after IUE with FUCCI cell cycle markers and control shRNA (A; N = 4 brains), F10 shRNA (B; N = 3), F10 and CAG-driven WT VRK1 (C; N = 3), F10 and CAG-driven VRK1–K179E (D; N = 3). Top, Merged image of: Middle, green channel, mVenus-hGeminin (S,G2,M)-positive cells and Bottom, red channel, mCherry-hCdt1(G1)-positive cells. A′–D′, The distribution of the proportion of cells expressing each of the FUCCI reporters (counted in separate channels) is represented in circular graphs. Red indicates mCherryCdt1+ (G1) cells. Green indicates mVenus-hGeminin+ (S/G2/M) cells. Yellow indicates that both markers are expressed (G1-to-S transition). Asterisks indicate significant changes compared with control (*p < 0.005). E, Schematic presentation showing the appearance of FUCCI chimeric markers during the cell cycle. CP, cortical plate; IZ, intermediate zone; VZ/sVZ, ventricular and subventricular zones.