Neurons in layer 5 projecting to both the cervical and lumbar cord in juvenile and adult mice. A, B, Representative coronal images of C7-projecting (green) and L4-projecting (red) neurons in layer 5 in the juvenile (A) and adult (B) primary motor cortex. Double-labeled neurons (yellow; arrowheads) were observed in both groups. C, D, Distribution of C7- and L4-double-projecting neurons in the juvenile (C) and adult (D) brain. Each distribution map was made using data pooled from 5 mice. Scale bars: A, B, 200 μm.