Figure 3.

Use-dependent activation persists in Kv1.2-containing heteromeric channel complexes with other Kv1 channel subtypes. A–C, Dimeric channel constructs were built with Kv1.2 and various other Kv1 channel subtypes, with protomers arranged in both orientations. Use-dependent activation was measured with trains of repetitive depolarizations (10 ms, 60 mV, 20 Hz), as described in Figures 3 and 4. D–F, Cell lysates were probed with a monoclonal anti-Kv1.2 antibody to confirm and validate synthesis of full-length dimer constructs (LM cells indicate mock transfected cells).