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. 2015 Feb 25;35(8):3701–3710. doi: 10.1523/JNEUROSCI.4160-14.2015

Figure 4.

Figure 4.

Re-acidification kinetics of different SV populations. A, Luminal pH of SVs endocytosed during varying stimulus-to-quench intervals (Δt). After stimulus, a 15 s acid quench was applied after Δt of 4 s (n = 9 experiments with 112 boutons), 8 s (n = 9 experiments with 129 boutons), 12 s (n = 10 experiments with 145 boutons), and 16 s (n = 10 experiments with 131 boutons). pHs of the endocytosed vesicle fraction were calculated as described in Figure 2. B, Comparison of the re-acidification kinetics measured with different Δt. The time constants were ∼15 s regardless of Δt (p = 0.96, ANOVA). C, pHvs of endocytosed fraction at the onset of poststimulus acid quench (pHo) plotted against Δt. A single-exponential fitting yielded a time constant of 17.8 s. Error bars indicate SEM.