Figure 9.

Strychnine does not inhibit responses induced by ectopic ChR2. A, Gr64f>ChR2 flies were stimulated with a 2 s pulse of BL and concurrently with different concentrations of strychnine. We analyzed the number of spikes (mean ± SEM) during the first second of the recordings in i9 (white diamonds; n = 7) and l5-l7 (black triangles; n = 11) sensilla in the presence of different concentrations of strychnine delivered sequentially in an increasing order of concentrations (0, 1, 5, and 10 mm), followed by BL only [0(R)] and nothing [0(off)]. These data were analyzed with a one-way ANOVA with Fisher's post hoc analysis. No effect of strychnine concentration on the response to BL was observed. Significant differences between 0(off) and 0, 1, 5, 10, and 0(R) were observed for i9 and l5/l7; however, only the interaction between 0(off) and 0 is shown on graph. B, As a control, we verified that Gr64f>ChR2 flies respond to sugar and that these responses are inhibited by strychnine. Black triangles, l5-l7 sensilla (n = 11); white diamonds, i9 sensilla (n = 9). stry, Strychnine; suc, sucrose; *p < 0.05; ***p < 0.001.