Figure 5.

DBZ regulates Ndel1 phosphorylation, and the phosphorylation-status of Ndel1 is critical for radial migration. A, Cell lysates from the cerebral cortices of E18.5 DBZ KO and WT were subjected to Western blot analyses. The phosphorylation of threonine 219 and serine 251 of Ndel1 increased in the DBZ KO. B, Radial migration was impaired by the ectopic expression of a dual-phospho-mimetic form of Ndel1 (T219E:S251E Ndel1). Scale bar, 100 μm. C, The ratio of cells with morphologically abnormal nuclei increased in T219E:S251E Ndel1-overexpressing cells. T219E:S251E Ndel1- or T219A:S251A Ndel1-expressing vectors were transfected into HeLa cells. Forty-eight hours later, the cells were immunostained with anti-tyrosinated tubulin antibody and observed with confocal microscopy. Scale bar, 5 μm. D, The ratio of cells with morphologically abnormal nucleus is shown. Values represent the mean ± SEM; *p < 0.05.