Cdk5 and Aurora A are involved in Ndel1 phosphorylation. A, B, Examination of the effect of GST-DBZ on phosphorylation of GST-Ndel1 by GST-Cdk5/p35 (A) and GST-Aurora A (B). GST-Ndel1 was incubated with GST-DBZ and GST-Cdk5/p35 (A) or GST-Aurora A (B). Phosphorylation of GST-Ndel1 decreased in the presence of DBZ (top) in both cases. CBB staining (bottom). C, Coimmunoprecipitation assay of DBZ and Ndel1. HEK293T cells were transfected with GFP-DBZ in combination with full-length Ndel1; myc-Ndel1-FL-FLAG (Ndel1-FL), the N-terminus of Ndel1; myc-Ndel1-Nt (Ndel1-Nt, residues 1–205), or the C-terminus of Ndel1; Ndel1-Ct-FLAG (Ndel1-Ct, residues 206–345). Immunoprecipitation was performed with the anti-GFP antibody, and Western blotting was performed with anti-myc and anti-FLAG antibodies.