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. 2015 Feb 11;35(6):2452–2464. doi: 10.1523/JNEUROSCI.4088-14.2015

Figure 3.

Figure 3.

MCP-1 and Siglec-9 are essential for SHED-CM-mediated M2-like macrophages induction. A, Quantification of MCP-1, Siglec-9, and IL-6 in CMs. B, Immunoblot analysis with an ED-Siglec-9-specific antibody. Full, Full-length Siglec-9; ED, ectodomain of Siglec-9. C, D, In vitro M2 induction assay with rat primary BBMs. C, Representative images of BMMs treated with DMEM, BMSC-CM, SHED-CM, or SHED-CM with three different antibodies or sialidase. Top, Optical-microscopy images; bottom, CD206/CD68/DAPI immunostaining. D, qPCR analysis of Cd206 and IL-10 mRNA expression. Results are relative to DMEM treatment. Sialidase treatment suppressed the SHED-CM-mediated M2 induction. ANOVA with Tukey's post hoc test (n = 3 separate experiments). Scale bar: C, 50 μm. Mean ± SD (A) and mean ± SEM (D). **p < 0.01; ***p < 0.001.