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. 2015 Feb 11;35(6):2452–2464. doi: 10.1523/JNEUROSCI.4088-14.2015

Figure 5.

Figure 5.

MCP-1/ED-Siglec-9-induced M2 exhibits neurorepairing and neuroprotective activities in vitro. A, Rat primary CGNs were placed on PLL-coated or PLL/CSPG-coated (300 ng/ml) plates and incubated for 24 h with serum-free DMEM, SHED-CM, 100 ng/ml MCP-1/ED-Siglec-9, or rat BMM-CMs and stained for tubulin or TUNEL. BMM-CMs were prepared by culturing in each condition (DMEM, IL-4, or MCP-1/ED-Siglec-9). B, C, Quantification of CGN neurite length and TUNEL+ apoptotic cells. Notably, CGNs plated with BMM-CM harvested from IL-4 or MCP-1/ED-Siglec-9-induced M2-like cells significantly extended their neurites and showed suppressed apoptosis in CSPG-coated wells, whereas the direct addition of MCP-1/ED-Siglec-9 to CGNs did not cause these effects. D, Quantification of the mRNA expression of the indicated genes in rat BMMs. M2-like macrophages induced by MCP-1/ED-Siglec-9 showed significantly increased expressions of neurotrophic/growth factors, such as Bdnf, hepatocyte growth factor (Hgf), and Vegf. ANOVA with Tukey's post hoc test (n = 3 separate experiments). Scale bar: A, 50 μm. Mean ± SD (B, C) and mean ± SEM (D). *p < 0.05; **p < 0.01; ***p < 0.001.