Figure 3.

The activity of OFC neurons can be conditioned by optogenetic stimulation of DRN Pet-1 neurons. A, ePet1-DRN^ChR2 mice were trained by coupling Odor 1 but not Odor 2 with delayed light stimulation of DRN Pet-1 neurons at 20 Hz for 2 s. OFC neurons were recorded after training animals for 400 trials over 2 d. The raster plot and peristimulus time histogram illustrate one OFC neuron that exhibited much higher activity during the delay period following the CS+ odor. B, Another OFC neuron showed strong inhibition following the delivery of the CS+ odor. C, One OFC neuron from a control ePet1-DRN^mCherry mouse did not show response in either the CS+ or CS− trial.