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. 2015 Feb 11;35(6):2657–2673. doi: 10.1523/JNEUROSCI.2484-14.2015

Figure 2.

Figure 2.

Existing OSNs through the nasal septum are disrupted by methimazole administration but recover to preinjury states within 28 d. A, Time course of the experimental design after methimazole (a) and saline (b) administration. Methimazole and saline were administered by intraperitoneal (i.p.) injections on day 0. Fixation (fix.) was performed on days 3 (d3), 7 (d7), 14 (d14), and 28 (d28) after methimazole administration and on day 28 (d28) after saline administration. B, Photomicrographs of representative coronal sections through the nasal septum on days 3, 7, 14, and 28 after methimazole administration. Top, Lower magnification; bottom, higher magnification views of the portion of the OE in top photographs outlined by the square. Left side of the OE in the image corresponds to the left nostril and the right side of the OE image corresponds to the right nostril. Scale bar, 100 μm at lower magnification, 20 μm at higher magnification. C–E, Thickness of the OE (C), number of OSNs (D), and number of supporting cells (E) on days 3, 7, 14, and 28 after methimazole administration and on day 28 after saline administration. On day 28 after methimazole administration, the thickness of the OE and the number of OSNs are restored to those levels observed after saline administration (n.s., not significant; Mann–Whitney test). F, Photomicrographs of representative coronal sections stained with anti-OMP antibody through the nasal septum 3, 7, 14, and 28 d after methimazole administration, and 28 d after saline administration. Left images, left nostril; right images, right nostril. Scale bar, 20 μm. G, Numbers of OMP-positive cells on both left and right sides on days 3, 7, 14, and 28 after methimazole administration and on day 28 after saline administration. On day 14 after methimazole-induced injury, the number of OMP-positive cells is significantly reduced compared with that on day 28 after saline administration (***p < 0.001, Mann–Whitney test), whereas, on day 28 after methimazole administration, the number of OMP-positive cells is restored to that observed on day 28 after saline administration (n.s., not significant; Mann–Whitney test).