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. 2015 Feb 11;35(6):2657–2673. doi: 10.1523/JNEUROSCI.2484-14.2015

Figure 4.

Figure 4.

Sensory deprivation results in the projection of axons from fewer newly generated OSNs. A, Analysis area of the OB. Left, coronal section of the OB. Square shows seven analyzed glomeruli in the OB. Scale bar, 500 μm. Middle, representative images of the left and right OB captured at day 28 after saline administration. Right, Intensity histograms of the OMP-immunostained area within glomeruli on both left and right sides. OMP-immunostained areas are shown in red for the left side and in blue for the right side of the OB. B, Representative coronal sections stained with anti-OMP antibody in the open (left) and the occluded (right) sides at days 3 (d3), 7 (d7), 14 (d14), and 28 (d28) after methimazole-induced injury and unilateral nostril occlusion. Each circled area corresponds to a glomerulus. Scale bar, 50 μm. C, Summary for the ratio of areas immunostained and unstained with OMP. The percentage of OMP-stained areas on days 3, 7, 14, and 28 after methimazole-induced injury and unilateral nostril occlusion (injury + n.o.) and on days 14 and 28 after methimazole-induced injury (injury only). OMP-stained area on the occluded side is significantly reduced compared with that on the open side on days 14 and 28 after methimazole-induced injury with unilateral nostril occlusion (*p < 0.05, ***p < 0.001; Mann–Whitney test). However, the OMP-immunostained area does not differ for these two conditions on days 14 and 28 (Mann–Whitney test). D, Schematic showing the quadrants of a coronal section through the OB (dorsolateral, d-l; dorsomedial, d-m; ventrolateral, v-l; and ventromedial, v-m). Scale bar, 500 μm. E, Representative coronal sections of each OB quadrant stained with anti-OMP antibody on the open (left) and the occluded (right) sides on day 28 (d28) after methimazole-induced injury and unilateral nostril occlusion (injury + n.o.). Each circled area corresponds to a glomerulus. Top, Dorsolateral quadrant (d-l); middle, dorsomedial quadrant (d-m); bottom, ventrolateral quadrant (v–l). Scale bar, 50 μm. F, Summary for the ratio of areas immunostained and unstained with OMP in each quadrant of the OB. The percent of the OMP-stained areas in each quadrant of the occluded side is significantly bottom than that of the open side (***p < 0.001; Mann–Whitney test).