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. 2015 Feb 11;35(6):2657–2673. doi: 10.1523/JNEUROSCI.2484-14.2015

Figure 7.

Figure 7.

Caspase-3-activated immature cells increase 14 and 28 d after methimazole-induced injury combined with nostril occlusion. A, Number of caspase-3-positive cells through the nasal septum on the open and occluded sides at days 7 (d7), 14 (d14), and 28 (d28) after unilateral nostril occlusion. Nostril occlusion does not induce a significant change in number of caspase-3-activated cells between the open and occluded sides at any time point (n.s., not significant; Mann–Whitney test). B, Number of caspase-3-positive cells through the nasal septum on days 7, 14, and 28 after injury. Methimazole administration does not induce a significant change in the number of caspase-3-activated cells between the left and right sides at any time (n.s., not significant; Mann–Whitney test). C, Coronal sections through the nasal septum stained with anti-caspase-3 antibody in the open and occluded sides at days 7, 14, and 28 after methimazole-induced injury with unilateral nostril occlusion. Scale bar, 20 μm. D, Number of caspase-3-positive cells through the nasal septum in the open and occluded sides on days 7, 14, and 28 after methimazole-induced injury with unilateral nostril occlusion. On days 14 and 28, the total number of caspase-3-positive cells on the occluded side is significantly higher than that on the open side (***p < 0.001; Mann–Whitney test). E, Coronal sections through the nasal septum stained with anti-caspase-3 (red) antibody and anti-OMP (green) antibody from the occluded side 14 d after methimazole-induced injury combined with unilateral nostril occlusion. Two representative images of the OE are shown in the top and bottom. The middle row of images shows a higher magnification of the OE captured from the boxed regions shown in the top row of images. White arrowheads indicate caspase-3-positive apoptotic cells that are not costained with an anti-OMP antibody, whereas the yellow arrowhead indicates a caspase-3-positive apoptotic cell costained with the anti-OMP antibody. A majority of caspase-3-positive cells were not costained with the anti-OMP antibody. Scale bar, 20 μm.