Figure 7.
Infusion of rapamycin does not affect phosphorylated levels of Ser845 at the PSD or at extrasynaptic sites. Rats were trained in an auditory fear-conditioning task and received an infusion of GluA23Y and rapamycin (GluA23Y/Rap; n = 12), scrambled-GluA23Y and rapamycin (Scr/Rap; n = 17), GluA23Y and DMSO (GluA23Y/DMSO; n = 12), or vehicle (Scr/DMSO; n = 13). A, C, Results show no significant correlation between the percentage of time spent freezing in the retrieval test and phosphorylated levels of Ser845 at the PSD (A) or extrasynaptic sites (C). B, D, Phosphorylated levels of Ser845 were equivalent in all groups at the PSD (B) and at extrasynaptic sites (D). Separate Western blot pictures in B come from the same gel. Levels of Ser845 are expressed as relative percentage values of the vehicle group ± SEM.