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. 2019 May 30;44(2):491–502. doi: 10.3892/ijmm.2019.4215

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Copyright: © Moon et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Inhibition of RhoA inhibits HSC activation and F-actin formation. HSC-T6 cells were pretreated with or without 10 µM Y27632 and 1 µg/ml Tat-C3 for 1 h, and 10 ng/ml TGF-β1 was added for 1 h. (A) Levels of α-SMA, p-cofilin and Col1a1 were assessed by western blotting and (B) quantified (mean ± SEM, n=3, ^*P<0.05, ^**P<0.01 and ^***P<0.001, one-way ANOVA, Tukey's post hoc test). (C) Immunocytochemical staining for F-actin formation in HSC-T6 cells using Alexa Fluor 488-phalloidin (green). DAPI (blue) was used to counterstain the nuclei. All images are representative of multiple images from three independent experiments (scale bar=20 µm). TGF-β1, transforming growth factor-β1; Col1a1, collagen type 1; α-SMA, α-smooth muscle actin; p-, phosphorylated; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; CON, control.

Inhibition of RhoA inhibits HSC activation and F-actin formation. HSC-T6 cells were pretreated with or without 10 µM Y27632 and 1 µg/ml Tat-C3 for 1 h, and 10 ng/ml TGF-β1 was added for 1 h. (A) Levels of α-SMA, p-cofilin and Col1a1 were assessed by western blotting and (B) quantified (mean ± SEM, n=3, ^*P<0.05, ^**P<0.01 and ^***P<0.001, one-way ANOVA, Tukey's post hoc test). (C) Immunocytochemical staining for F-actin formation in HSC-T6 cells using Alexa Fluor 488-phalloidin (green). DAPI (blue) was used to counterstain the nuclei. All images are representative of multiple images from three independent experiments (scale bar=20 µm). TGF-β1, transforming growth factor-β1; Col1a1, collagen type 1; α-SMA, α-smooth muscle actin; p-, phosphorylated; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; CON, control.