Figure 2.

TMP attenuates H₂O₂-induced oxidative damage in PRGCs. PRGCs were pre-treated with 25, 50 and 100 µM of TMP for 24 h prior to being exposed to 400 µM of H₂O₂. (A and B) ROS production was measured by a 2′,7′-dichlorofluorescin-diacetate assay (magnification, ×200). (C and D) The levels of MDA and SOD were detected by an ELISA assay. Data are presented as the mean of three replicates ± standard deviation. ^*P<0.05, ^**P<0.01 vs. Control group, ^#P<0.05, ^##P<0.01 vs. Vehicle + H₂O₂ group. PRGCs, primary retinal ganglion cells; MDA, malondialdehyde; SOD, superoxide dismutase; TMP, tetramethylpyrazine.