Figure 5.

BNIP3 is a direct target of miR-182. (A) The putative binding site of miR-182 and BNIP3; (B) a luciferase assay of 293 co-transfected with firefly luciferase constructs containing the BNIP3 wt or mut 3′-UTR and miR-182 mimics, mimic NC, miR-182 inhibitor or inhibitor NC (n=3). Data are presented as the mean of three replicates ± standard deviation. ^**P<0.01 vs. mimics NC, ^##P<0.01 vs. inhibitor NC. (C) The expression of BNIP3 protein after transfection with miR-182 mimic or miR-182 inhibitor, as measured by western blotting. β-actin was used as a loading control. BNIP3, Bcl-2 interacting protein 3; miR, microRNA; mut, mutated; NC, negative control; UTR, untranslated region; wt, wild-type.